Human KCND3 (Potassium voltage-gated channel subfamily D member 3) ELISA Kit

Human KCND3 (Potassium voltage-gated channel subfamily D member 3) ELISA KitThe Human KCND3 (Potassium voltage gated channel subfamily D member 3) ELISA Kit measures KCND3 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti KCND3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound

The Human KCND3 (Potassium voltage-gated channel subfamily D member 3) ELISA Kit measures KCND3 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti KCND3 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with KCND3 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of KCND3 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.